Where common restriction enzymes cut the — bp plasmid. The search runs on the circular sequence, so sites spanning the origin are found too. Click a row to see the sites on a linear map; the link next to it shows them in the sequence itself.
| Enzyme | Site | Sites | Cut positions |
|---|
A unique site is where the vector can be cut exactly once without breaking it into pieces — that is how a plasmid is linearised before an insert goes in. In the LIC cloning this vector is built for, the cut vector is then treated with T4 DNA polymerase, which chews back long single-stranded ends; those simply anneal with the insert, no ligase needed. A digest is also the cheapest way to check that a colony carries the right plasmid: the pattern of fragment lengths on the gel must match the prediction.