Addgene #29747, — bp: a His6–MBP–TEV site–mCherry cassette under the T7 promoter. Click a feature on the map — the coordinates fill in and the fragment is highlighted.
| Resistance | kanamycin (KanR) |
| Promoter | T7 + lac operator — IPTG induction in BL21(DE3) |
| Tags | His6 → Ni-NTA; MBP → amylose resin |
| Tag removal | TEV protease cuts ENLYFQ↓S |
| Origin | ColE1/pMB1, high copy; f1 ori also present |
| Feature | Position | bp | Strand |
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